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Mouse- and Human-derived Primary Gastric Epithelial Monolayer Culture for the Study of Regeneration
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JoVE 杂志 免疫与感染
Mouse- and Human-derived Primary Gastric Epithelial Monolayer Culture for the Study of Regeneration

Mouse- and Human-derived Primary Gastric Epithelial Monolayer Culture for the Study of Regeneration

DOI:
10.3791/57435-v

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11:48 min

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May 07, 2018

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, , , ,
1Department of Pharmacology and Systems Physiology,University of Cincinnati, 2Department of Cell & Developmental Biology,University of Michigan, 3Department of Molecular Genetics, Biochemistry & Microbiology,University of Cincinnati

Chapters

  • 00:04Title
  • 01:04Establishing Human-derived 3D Gastric Fundic Organoids
  • 05:23Transfer of 3D m/hFGOs to 2D Gastric Epithelial Cell Monolayers
  • 07:44Scratch Wound Assay Using 2D Gastric Epithelial Cell Monolayers
  • 09:12Results: Use of Gastric Epithelial Monolayer as a Scratch-wound Assay for Gastric Regeneration
  • 10:53Conclusion

Summary

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Here we describe a protocol for establishing and culturing human- and mouse-derived 3-dimensional (3D) gastric organoids, and the method for the transfer of 3D organoids to a 2-dimensional monolayer. The use of the gastric epithelial monolayer as a novel scratch-wound assay for regeneration studies is also described.

Tags

Gastric Epithelial MonolayerPrimary Cell CultureMouseHumanRegenerationWound HealingHelicobacter PyloriEpithelial Immune Cell Co-culturePrimary Gastric CellsOrganoid Generation

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