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Isolation and RNA Extraction of Neurons, Macrophages and Microglia from Larval Zebrafish Brains
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Isolation and RNA Extraction of Neurons, Macrophages and Microglia from Larval Zebrafish Brains

Isolation and RNA Extraction of Neurons, Macrophages and Microglia from Larval Zebrafish Brains

13,193 Views

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09:20 min

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April 27, 2018

DOI:

10.3791/57431-v

DOI:
10.3791/57431-v

•

09:20 min
April 27, 2018

•

1 Views
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1Centre for Discovery Brain Sciences,University of Edinburgh

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IsolationRNA ExtractionNeuronsMacrophagesMicrogliaLarval Zebrafish BrainsTransgenic ZebrafishLive ImagingGene ExpressionCell SortingCentral Nervous SystemPTUFluorescent StereomicroscopeGFPDsREDTricaineHomogenizerMedia ARNA PurificationQPCRTranscriptomics

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Mazzolini, J., Chia, K., Sieger, D. Isolation and RNA Extraction of Neurons, Macrophages and Microglia from Larval Zebrafish Brains. J. Vis. Exp. (134), e57431, doi:10.3791/57431 (2018).

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