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Isolation and RNA Extraction of Neurons, Macrophages and Microglia from Larval Zebrafish Brains
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Isolation and RNA Extraction of Neurons, Macrophages and Microglia from Larval Zebrafish Brains
Capitoli
- 00:04Titolo
- 01:05Homogenization
- 04:40Microglia Immunostaining
- 06:41Results: Isolation of Neurons, Macrophages, and Microglia, and their RNA from Larval Zebrafish Brains
- 08:25Conclusion
We present a protocol to isolate neurons, macrophages and microglia from larval zebrafish brains under physiological and pathological conditions. Upon isolation, RNA is extracted from these cells to analyze their gene expression profile. This protocol allows for the collection of high-quality RNA for performing downstream analysis like qPCR and transcriptomics.
