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Live Cell Imaging della segregazione cromosomica durante la mitosi
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发育生物学
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JoVE 杂志 发育生物学
Live Cell Imaging of Chromosome Segregation During Mitosis
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Live Cell Imaging della segregazione cromosomica durante la mitosi

9,073 Views

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06:39 min

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March 14, 2018

DOI:

10.3791/57389-v

DOI:
10.3791/57389-v

•

06:39 min
March 14, 2018

•

7 Views
, ,
1Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research,US Food and Drug Administration, 2Microscopy and Imaging Core facility, Division of Viral Products, Center for Biologics Evaluation and Research,US Food and Drug Administration

Summary

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Questo protocollo descrive un metodo facile e conveniente per etichettare e visualizzare live cromosomi nelle cellule mitotiche utilizzando etichetta Histone2B-GFP BacMam 2.0 e un sistema di microscopia confocal di filatura disco.

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Cite this Article

Varadkar, P., Takeda, K., McCright, B. Live Cell Imaging of Chromosome Segregation During Mitosis. J. Vis. Exp. (133), e57389, doi:10.3791/57389 (2018).

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