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Generation of Large Numbers of Myeloid Progenitors and Dendritic Cell Precursors from Murine Bone Marrow Using a Novel Cell Sorting Strategy
Chapters
- 00:04Title
- 00:40Collection of Murine Bone Marrow Cells
- 03:57Staining Strategy
- 05:53Set Gates Based on Control Samples
- 06:22Collection of Isolated Populations
- 07:20Results: Robust Myeloid Progenitors Using Protocol
- 08:43Conclusion
Here we provide a method for identifying and isolating large numbers of GM-CSF driven myeloid cells using high speed cell sorting. Five distinct populations (Common myeloid progenitors, granulocyte/macrophage progenitors, monocytes, monocyte-derived macrophages, and monocyte-derived DCs) can be identified based on Ly6C and CD115 expression.
