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A Murine Pancreatic Islet Cell-based Screening for Diabetogenic Environmental Chemicals
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A Murine Pancreatic Islet Cell-based Screening for Diabetogenic Environmental Chemicals

A Murine Pancreatic Islet Cell-based Screening for Diabetogenic Environmental Chemicals

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07:39 min

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June 25, 2018

DOI:

10.3791/57327-v

DOI:
10.3791/57327-v

•

07:39 min
June 25, 2018

•

2 Views
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1Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization,Hunan Agriculture University, 2Texas A&M University, 3Texas A&M Institute for Genomic Medicine, 4Sun Yat-Sen Memorial Hospital

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Here we present a protocol to isolate mouse pancreatic islet cells for screening the ROS inductions by the xenobiotics in order to identify the potential diabetogenic xenobiotic chemicals.

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PancreasIslet CellsROSType-2 DiabetesIn VitroCollagenaseDigestionIsolationPurificationGlucose-stimulated Insulin Secretion

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Chen, J., Zhong, L., Wu, J., Ke, S., Morpurgo, B., Golovko, A., Ouyang, N., Sun, Y., Guo, S., Tian, Y. A Murine Pancreatic Islet Cell-based Screening for Diabetogenic Environmental Chemicals. J. Vis. Exp. (136), e57327, doi:10.3791/57327 (2018).

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