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A Bioluminescent and Fluorescent Orthotopic Syngeneic Murine Model of Androgen-dependent and Castration-resistant Prostate Cancer
JoVE 杂志
癌症研究
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JoVE 杂志 癌症研究
A Bioluminescent and Fluorescent Orthotopic Syngeneic Murine Model of Androgen-dependent and Castration-resistant Prostate Cancer

A Bioluminescent and Fluorescent Orthotopic Syngeneic Murine Model of Androgen-dependent and Castration-resistant Prostate Cancer

12,508 Views

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07:25 min

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March 06, 2018

DOI:

10.3791/57301-v

DOI:
10.3791/57301-v

•

07:25 min
March 06, 2018

•

5 Views
, , , ,
1Department of Urology,Northwestern University Feinberg School of Medicine, 2The Robert H. Lurie Comprehensive Cancer Center,Northwestern University Feinberg School of Medicine, 3Department of Pathology,Northwestern University Feinberg School of Medicine

Summary

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The goal of this protocol is to demonstrate the intra-prostatic injection of prostate cancer cells, with subsequent castration. Orthotopic pre-clinical models of androgen-dependent and castration-resistant prostate cancer are critical to study the disease in the context of a clinically relevant tumor microenvironment and an immunocompetent host.

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Anker, J. F., Mok, H., Naseem, A. F., Thumbikat, P., Abdulkadir, S. A. A Bioluminescent and Fluorescent Orthotopic Syngeneic Murine Model of Androgen-dependent and Castration-resistant Prostate Cancer. J. Vis. Exp. (133), e57301, doi:10.3791/57301 (2018).

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