Journal
/
发育生物学
/
Identification of Skeletal Muscle Satellite Cells by Immunofluorescence with Pax7 and Laminin Antibodies
JoVE 杂志
发育生物学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 发育生物学
Identification of Skeletal Muscle Satellite Cells by Immunofluorescence with Pax7 and Laminin Antibodies

Identification of Skeletal Muscle Satellite Cells by Immunofluorescence with Pax7 and Laminin Antibodies

DOI:
10.3791/57212-v

•

07:18 min

•

April 19, 2018

•

, , , ,
1Laboratory of Muscle Stem Cells and Gene Regulation,National Institutes of Health, 2Office of Science and Technology, National Institute of Arthritis, Musculoskeletal and Skin Diseases (NIAMS),National Institutes of Health

Chapters

  • 00:05Title
  • 00:41Cryo-embedding of the Tibialis Anterior Muscle
  • 01:46Cryostat Sectioning
  • 02:33Immunofluorescence and Staining
  • 05:27Results: Visualization of Satellite Cells in Adult Resting Muscle Sections is Improved Using Immunofluorescence-based Staining
  • 06:40Conclusion

Summary

自动翻译

自动翻译

The precise identification of satellite cells is essential for studying their functions under various physiological and pathological conditions. This article presents a protocol to identify satellite cells on adult skeletal muscle sections by immunofluorescence-based staining.

Tags

Skeletal MuscleSatellite CellsImmunofluorescencePax7LamininCryosectioningCryostatAntigen RetrievalImmunostaining

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

Isolation and Quantitative Immunocytochemical Characterization of Primary Myogenic Cells and Fibroblasts from Human Skeletal Muscle

Isolation and Characterization of Satellite Cells from Rat Head Branchiomeric Muscles

Analysis of Zebrafish Larvae Skeletal Muscle Integrity with Evans Blue Dye

Induction of Acute Skeletal Muscle Regeneration by Cardiotoxin Injection

Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans

Integration Free Derivation of Human Induced Pluripotent Stem Cells Using Laminin 521 Matrix

Application of Chronic Stimulation to Study Contractile Activity-induced Rat Skeletal Muscle Phenotypic Adaptations

Isolation, Culture, Characterization, and Differentiation of Human Muscle Progenitor Cells from the Skeletal Muscle Biopsy Procedure

Performing Human Skeletal Muscle Xenografts in Immunodeficient Mice

Isolation and Differentiation of Primary Myoblasts from Mouse Skeletal Muscle Explants

Read Article