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Variations on Negative Stain Electron Microscopy Methods: Tools for Tackling Challenging Systems
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
Variations on Negative Stain Electron Microscopy Methods: Tools for Tackling Challenging Systems

Variations on Negative Stain Electron Microscopy Methods: Tools for Tackling Challenging Systems

31,192 Views

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06:06 min

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February 06, 2018

DOI:

10.3791/57199-v

DOI:
10.3791/57199-v

•

06:06 min
February 06, 2018

•

28 Views
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1Astbury Centre for Structural Molecular Biology,University of Leeds, 2Astbury Biostructure Laboratory,University of Leeds

Tags

Negative StainElectron MicroscopyGrid PreparationCarbon SheetGlow DischargeSide BlotFlickingStaining ReagentsSample AdsorptionCapillary ActionAir Drying

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Scarff, C. A., Fuller, M. J. G., Thompson, R. F., Iadanza, M. G. Variations on Negative Stain Electron Microscopy Methods: Tools for Tackling Challenging Systems. J. Vis. Exp. (132), e57199, doi:10.3791/57199 (2018).

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