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A Comprehensive Procedure to Evaluate the In Vitro Performance of the Putative Hemangioblastoma Neovascularization Using the Spheroid Sprouting Assay
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Cancer Research
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JoVE Journal Cancer Research
A Comprehensive Procedure to Evaluate the In Vitro Performance of the Putative Hemangioblastoma Neovascularization Using the Spheroid Sprouting Assay

A Comprehensive Procedure to Evaluate the In Vitro Performance of the Putative Hemangioblastoma Neovascularization Using the Spheroid Sprouting Assay

DOI:
10.3791/57183-v

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08:26 min

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April 12, 2018

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, , , , ,
1Department of Neurosurgery, Huashan Hospital,Fudan University

Chapters

  • 00:04Title
  • 01:46Cell Culture and Plasmid Construct
  • 03:00Lentivirus Package and Infection
  • 04:19Generation of Modified Endothelial Cell Spheroids
  • 05:04In Vitro Angiogenesis Assay
  • 06:26Results: Use of Spheroid Sprouting Assay to Study Modified Endothelial Cell Vessel Formation
  • 07:30Conclusion

Summary

Automatic Translation

Automatic Translation

This paper presents a comprehensive procedure to evaluate in vitro whether classic tumor angiogenesis exists in hemangioblastomas (HBs) and its role in HBs. The results highlight the complexity of HB-neovascularization and suggest that this common form of angiogenesis is only a complementary mechanism in the HB-neovascularization.

Tags

Keywords: Spheroid Sprouting AssayHemangioblastomaNeovascularizationTumor AngiogenesisVasculogenesisHUVECShRNALentiviral VectorEndothelial CellAngiogenesisTumor Vasculogenic Mimicry

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