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膜蛋白对洗涤剂敏感相互作用的检测
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生物化学
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JoVE 杂志 生物化学
Detection of Detergent-sensitive Interactions Between Membrane Proteins
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膜蛋白对洗涤剂敏感相互作用的检测

5,826 Views

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10:09 min

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March 07, 2018

DOI:

10.3791/57179-v

DOI:
10.3791/57179-v

•

10:09 min
March 07, 2018

•

2 Views
, ,
1Department of Biochemistry,Boston University School of Medicine

Summary

Automatically generated

我们描述了一个协议, 以检测在膜蛋白之间的洗涤剂敏感的相互作用, 使用结合的排序受体, sortilin, 到第一个腔内循环的葡萄糖转运蛋白, GLUT4, 作为一个例子。

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Cite this Article

Zaarur, N., Pan, X., Kandror, K. V. Detection of Detergent-sensitive Interactions Between Membrane Proteins. J. Vis. Exp. (133), e57179, doi:10.3791/57179 (2018).

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