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An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions
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遗传学
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JoVE 杂志 遗传学
An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions

An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions

7,508 Views

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07:59 min

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March 22, 2018

DOI:

10.3791/57149-v

DOI:
10.3791/57149-v

•

07:59 min
March 22, 2018

•

1 Views
,
1Institute for Frontier Life and Medical Sciences,Kyoto University, 2Japan Science and Technology Agency,PRESTO, 3Institute for Integrated Cell-Material Sciences,Kyoto University, 4Graduate School of Medicine,Kyoto University, 5Graduate School of Biostudies,Kyoto University

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Here, we present a protocol to analyze cell-to-cell transfer of oscillatory information by optogenetic control and live monitoring of gene expression. This approach provides a unique platform to test a functional significance of dynamic gene expression programs in multicellular systems.

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Cite this Article

Isomura, A., Kageyama, R. An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions. J. Vis. Exp. (133), e57149, doi:10.3791/57149 (2018).

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