00:49Fluorescence Labeling of Extracellular Vesicles
03:05Visualize Uptake of Extracellular Vesicles by Confocal Microscopy
06:18Endothelial Cell Permeability
07:36Determine Puromycin Sensitivity
08:59Transduction of Endothelial Cells with Lentiviral Vector
10:53Results: Use of Confocal Microscopy and Diffusion Capacity of Fluorochrome Dextran to Study the Interaction Between the Extracellular Vesicles and Endothelial Cells
I dette arbejde beskriver vi protokoller for at undersøge den rolle af ekstracellulære vesikler (EVs) udgivet af Plasmodium falciparum inficeret erytrocytter. Især fokuserer vi på samspillet mellem evt med endotelceller.