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Evaluation of Extracellular Vesicle Function During Malaria Infection
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JoVE 杂志 免疫与感染
Evaluation of Extracellular Vesicle Function During Malaria Infection

Evaluation of Extracellular Vesicle Function During Malaria Infection

DOI:
10.3791/57067-v

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13:45 min

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February 14, 2018

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, , , , ,
1Department of Medicine,University of Fribourg

Chapters

  • 00:04Title
  • 00:49Fluorescence Labeling of Extracellular Vesicles
  • 03:05Visualize Uptake of Extracellular Vesicles by Confocal Microscopy
  • 06:18Endothelial Cell Permeability
  • 07:36Determine Puromycin Sensitivity
  • 08:59Transduction of Endothelial Cells with Lentiviral Vector
  • 10:53Results: Use of Confocal Microscopy and Diffusion Capacity of Fluorochrome Dextran to Study the Interaction Between the Extracellular Vesicles and Endothelial Cells
  • 12:50Conclusion

Summary

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In this work, we describe protocols to investigate the role of extracellular vesicles (EVs) released by Plasmodium falciparum infected erythrocytes. In particular, we focus on the interactions of EVs with endothelial cells.

Tags

Extracellular VesiclesMalariaParasite-host CommunicationVesicular RNAPKH67 DyeEndothelial CellsCentrifugationStainingCell Culture

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