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1Department of Cellular and Integrative Physiology,Indiana University School of Medicine, 2Herman B Wells Center for Pediatric Research,Indiana University School of Medicine, 3Indiana Center for Biological Microscopy,Indiana University School of Medicine, 4Department of Medicine,Indiana University School of Medicine, 5Department of Pediatrics,Indiana University School of Medicine, 6Department of Microbiology and Immunology,Indiana University School of Medicine, 7Indiana University Simon Cancer Center,Indiana University School of Medicine
Chapters
- 00:05Title
- 00:59Microscope Preparation and Endothelial Colony Forming Cell (ECFC) Matrix Plating
- 04:46Image Acquisition and Processing and Kinetic Analysis of Vasculogenesis (KAV)
- 08:12Results: Representative Kinetic Analysis of Vasculogenesis
- 10:22Conclusion
Aquí, presentamos un protocolo para la proyección de imagen de Time-lapse y fase de análisis de la vasculogénesis en vitro usando microscopía de contraste junto con el software de código abierto, análisis cinético de la vasculogénesis. Este protocolo se puede aplicar para evaluar cuantitativamente el potencial de Vasculogénica de numerosos tipos de células o condiciones experimentales que modelo de enfermedad vascular.
