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Journal / Biochemistry / 小鼠肝灌注肝细胞和正弦内皮细胞的纯化…
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Biochemistry

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JoVE Journal Biochemistry
小鼠肝灌注肝细胞和正弦内皮细胞的纯化
 
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小鼠肝灌注肝细胞和正弦内皮细胞的纯化

Article DOI: 10.3791/56993-v • 08:54 min • February 12th, 2018
February 12th, 2018 •
1, 1, 1, 1, 1, 1, 1
1Department of Biochemistry, University of Nebraska

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该协议的目的是获得高生存能力和高产量的肝细胞和正弦内皮细胞。这是通过灌注肝与 IV 型胶原酶溶液通过门静脉, 其次是差异离心获得肝细胞和正弦内皮细胞。

Tags

生物化学 问题 132 肝脏灌注 肝细胞 正弦内皮细胞 小鼠肝脏 鼠肝 胶原酶 细胞纯化
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