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Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence
JoVE 杂志
免疫与感染
This content is Free Access.
JoVE 杂志 免疫与感染
Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence

Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence

DOI:
10.3791/56801-v

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10:06 min

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February 26, 2018

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, , ,
1Department of Microbial Pathogenesis and Immunology,Texas A&M Health Science Center, 2Tuberculosis Research Section, Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases,National Institutes of Health

Chapters

  • 00:04Title
  • 01:02Preparing the M. tuberculosis Inoculum
  • 03:13Inoculating Mice with Airborne M. tuberculosis
  • 05:33Reporter Enzyme Fluorescence Imaging and Analysis of Lung Homogenate
  • 08:05Results: Imaging
  • 09:32Conclusion

Summary

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We describe the optical imaging of mice infected with Mycobacterium tuberculosis (M. tuberculosis) using reporter enzyme fluorescence (REF). This protocol facilitates the sensitive and specific detection of M. tuberculosis in pre-clinical animal models for pathogenesis, therapeutics and vaccine research.

Tags

Reporter Enzyme FluorescenceMycobacterium TuberculosisPre-clinical Animal ModelsPathogenesisTherapeuticsVaccine ResearchVirulence MechanismsHost-pathogen InteractionsMOADCTW Medium7H11 PlatesCFUBiosafety HoodNebulizerInoculation Chamber

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