Journal
/
环境科学
/
Experimental Protocol for Detecting Mitochondrial Function in Hepatocytes Exposed to Organochlorine Pesticides
JoVE 杂志
环境科学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 环境科学
Experimental Protocol for Detecting Mitochondrial Function in Hepatocytes Exposed to Organochlorine Pesticides

Experimental Protocol for Detecting Mitochondrial Function in Hepatocytes Exposed to Organochlorine Pesticides

DOI:
10.3791/56800-v

•

08:39 min

•

September 16, 2020

•

, ,
1State Key Laboratory of Reproductive Medicine, Institute of Toxicology,Nanjing Medical University, 2Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health,Nanjing Medical University, 3Center of Gallbladder Disease, Shanghai East Hospital, Institute of Gallstone Disease,Tongji University School of Medicine

Chapters

  • 00:04Title
  • 00:36Mitochondrial Fluorescence Intensity and Cellular ATP Detection
  • 02:45Mitochondrial Membrane Potential Measurements
  • 04:39Oxygen Consumption Rate (OCR) Measurements
  • 06:22Results: Evaluation of Mitochondrial Function in Hepatocytes Exposed to β-HCH
  • 07:43Conclusion

Summary

自动翻译

自动翻译

Understanding the influence of environmental organochlorine pesticides (OCPs) on mitochondrial function in hepatocytes is important in exploring the mechanism of OCPs causing metabolic disorders. This paper presents detailed methods on detecting hepatic mitochondrial function.

Tags

Keywords: Mitochondrial FunctionHepatocytesOrganochlorine PesticidesATP LevelOxygen ConsumptionHepG2 CellsBeta-hexachlorcyclohexaneMitochondrial Green Fluorescence ProbeConfocal MicroscopyCellular ATPLuminometerMitochondrial Membrane PotentialJC1 Dye

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

check_url/cn/56800?article_type=v

Experimental Protocol for Manipulating Plant-induced Soil Heterogeneity
check_url/cn/56800?article_type=v

A Protocol for Conducting Rainfall Simulation to Study Soil Runoff
check_url/cn/56800?article_type=v

Administering and Detecting Protein Marks on Arthropods for Dispersal Research
check_url/cn/56800?article_type=v

LC-MS Analysis of Human Platelets as a Platform for Studying Mitochondrial Metabolism
check_url/cn/56800?article_type=v

Characterization and Application of Passive Samplers for Monitoring of Pesticides in Water
check_url/cn/56800?article_type=v

Experimental Protocol for Biodiesel Production with Isolation of Alkenones as Coproducts from Commercial Isochrysis Algal Biomass
check_url/cn/56800?article_type=v

Experimental Protocol for Detecting Cyanobacteria in Liquid and Solid Samples with an Antibody Microarray Chip
check_url/cn/56800?article_type=v

Extraction of Organochlorine Pesticides from Plastic Pellets and Plastic Type Analysis
check_url/cn/56800?article_type=v

Video Tracking Protocol to Screen Deterrent Chemistries for Honey Bees
check_url/cn/56800?article_type=v

Dispersion of Nanomaterials in Aqueous Media: Towards Protocol Optimization

Read Article