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Visualization of Biofilm Formation in Candida albicans Using an Automated Microfluidic Device
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Visualization of Biofilm Formation in Candida albicans Using an Automated Microfluidic Device

Visualization of Biofilm Formation in Candida albicans Using an Automated Microfluidic Device

DOI:
10.3791/56743-v

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11:14 min

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December 14, 2017

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, , ,
1Department of Molecular and Cell Biology, School of Natural Sciences,University of California, Merced

Chapters

  • 00:05Title
  • 01:02Preparation of Microfluidic Channels of the Microfluidic Plate
  • 02:08Candida albicans Biofilm Formation in the Microfluidic System
  • 04:06Setting Up the Stage Positions for the Microfluidic Experiment
  • 06:01Microfluidic Experiment Image Acquisition
  • 07:38Analyzing the Results
  • 09:07Results: Drug Exposure and Genetic Mutations Can Alter Biofilm Formation
  • 10:20Conclusion

Summary

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This protocol describes the use of a customizable automated microfluidic device to visualize biofilm formation in Candida albicans under host physiological conditions.

Tags

Biofilm FormationCandida AlbicansMicrofluidic DeviceReal-time VisualizationAntimicrobial AgentsGenetic MutantsHigh-throughput ScreeningYPD MediumTemperature ControlShear StressConstant Flow Mode

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