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Immobilization of Caenorhabditis elegans to Analyze Intracellular Transport in Neurons
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发育生物学
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JoVE 杂志 发育生物学
Immobilization of Caenorhabditis elegans to Analyze Intracellular Transport in Neurons

Immobilization of Caenorhabditis elegans to Analyze Intracellular Transport in Neurons

DOI:
10.3791/56690-v

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07:35 min

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October 18, 2017

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1Frontier Research Institute for Interdisciplinary Sciences and Graduate School of Life Sciences,Tohoku University

Chapters

  • 00:05Title
  • 00:34Experimental Preparation
  • 02:00Mounting of Worms
  • 02:56Imaging
  • 05:23Results: Visualization of Axonal and Intraflagellar Transport in Neurons
  • 06:53Conclusion

Summary

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自动翻译

Caenorhabditis elegans (C. elegans) is a good model to study axonal and intracellular transport. Here, I describe a protocol for in vivo recording and analysis of axonal and intraflagellar transport in C. elegans.

Tags

Caenorhabditis ElegansIntracellular TransportNeuronsAxonal TransportIntraflagellar TransportKinesinDyneinMicrotubulesGreen Fluorescent ProteinIn VivoLive ImagingAgarose Gel

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