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1Department of Physiology, School of Basic Medical Sciences,Southern Medical University, 2School of Basic Medical Sciences,Guizhou Medical University, 3Department of Laboratory Medicines, Guangzhou General Hospital of Guangzhou Military Region,Southern Medical University, 4Experiment Teaching Center, School of Basic Medical Sciences,Southern Medical University
Chapters
- 00:04Title
- 00:56Auditory Brainstem Response Recording
- 02:12The Organ of Corti Dissection
- 03:22Immunofluorescence Staining
- 05:21Patch Clamp Recording of Isolated OHCs
- 07:33Results: Whole-Cell Patch Clamp Recording of Isolated Outer Hair Cells
- 08:19Conclusion
Denne protokollen beskriver metoder for å registrere auditiv hjernestammen svaret fra postnatal rotte unger. For å undersøke funksjonelle utviklingen av ytre hårcellene, er eksperimentelle prosedyren for hele celle oppdateringen klemme i isolert ytre hårcellene beskrevet trinn for trinn.
