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Auditieve Brainstem Response en buitenste Haarcel geheel-cel Patch klem opname bij postnatale ratten
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Auditory Brainstem Response and Outer Hair Cell Whole-cell Patch Clamp Recording in Postnatal Rats
DOI:

09:23 min

May 24, 2018

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Chapters

  • 00:04Title
  • 00:56Auditory Brainstem Response Recording
  • 02:12The Organ of Corti Dissection
  • 03:22Immunofluorescence Staining
  • 05:21Patch Clamp Recording of Isolated OHCs
  • 07:33Results: Whole-Cell Patch Clamp Recording of Isolated Outer Hair Cells
  • 08:19Conclusion

Summary

自动翻译

Dit protocol wordt beschreven methoden om vast te leggen van de auditieve brainstem response van postnatale rat pups. Om te onderzoeken de functionele ontwikkeling van buitenste haarcellen, is de experimentele procedure van geheel-cel patch klem opnemen in geïsoleerde buitenste haarcellen stapsgewijze beschreven.

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