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Auditory Brainstem Response and Outer Hair Cell Whole-cell Patch Clamp Recording in Postnatal Rats
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JoVE 杂志 神经科学
Auditory Brainstem Response and Outer Hair Cell Whole-cell Patch Clamp Recording in Postnatal Rats

Auditory Brainstem Response and Outer Hair Cell Whole-cell Patch Clamp Recording in Postnatal Rats

DOI:
10.3791/56678-v

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09:23 min

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May 24, 2018

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, , , , ,
1Department of Physiology, School of Basic Medical Sciences,Southern Medical University, 2School of Basic Medical Sciences,Guizhou Medical University, 3Department of Laboratory Medicines, Guangzhou General Hospital of Guangzhou Military Region,Southern Medical University, 4Experiment Teaching Center, School of Basic Medical Sciences,Southern Medical University

Chapters

  • 00:04Title
  • 00:56Auditory Brainstem Response Recording
  • 02:12The Organ of Corti Dissection
  • 03:22Immunofluorescence Staining
  • 05:21Patch Clamp Recording of Isolated OHCs
  • 07:33Results: Whole-Cell Patch Clamp Recording of Isolated Outer Hair Cells
  • 08:19Conclusion

Summary

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This protocol describes methods to record the auditory brainstem response from postnatal rat pups. To examine the functional development of outer hair cells, the experimental procedure of whole-cell patch clamp recording in isolated outer hair cells is described step-by-step.

Tags

Auditory Brainstem ResponseOuter Hair CellWhole-cell Patch ClampPostnatal RatsCochlear Hair CellsAuditory FunctionElectrophysiologyInner EarCochleaOrgan Of CortiLeibovitz’s L-15 MediumDissection

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