Journal
/
神经科学
/
Modified Roller Tube Method for Precisely Localized and Repetitive Intermittent Imaging During Long-term Culture of Brain Slices in an Enclosed System
JoVE 杂志
神经科学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 神经科学
Modified Roller Tube Method for Precisely Localized and Repetitive Intermittent Imaging During Long-term Culture of Brain Slices in an Enclosed System

Modified Roller Tube Method for Precisely Localized and Repetitive Intermittent Imaging During Long-term Culture of Brain Slices in an Enclosed System

DOI:
10.3791/56436-v

•

09:52 min

•

December 28, 2017

•

, , , , , , , ,
1Department of Biochemistry and Molecular Biology and Molecular, Cellular and Integrated Neuroscience Program,Colorado State University, 2IBMC-Instituto de Biologia Molecular e Celular, i3S-Instituto de Investigaçãoe Inovação em Saúde, ICBAS,Universidade do Porto, 3Denali Therapeutics

Chapters

  • 00:05Title
  • 01:28Preparation of Roller Tubes and Coverslips
  • 02:51Plating Slices
  • 05:44Slice Imaging
  • 07:28Results: Repetitive Intermittent Imaging of Brain Slices During Long-term Culture
  • 08:49Conclusion

Summary

自动翻译

自动翻译

Presented here is a modified roller tube method for culturing and intermittent high-resolution imaging of rodent brain slices over many weeks with precise repositioning on photoetched coverslips. Neuronal viability and slice morphology are well maintained. Applications of this fully enclosed system using viruses for cell-type specific expression are provided.

Tags

Brain Slice CultureLong-term CultureRepetitive ImagingFluorescence ImagingEnclosed SystemRoller Tube MethodPhoto Etched CoverslipsViral VectorsNeurological DisordersSynapse AlterationRodent Models

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

A Polished and Reinforced Thinned-skull Window for Long-term Imaging of the Mouse Brain

A Molecular Readout of Long-term Olfactory Adaptation in C. elegans

Improved Preparation and Preservation of Hippocampal Mouse Slices for a Very Stable and Reproducible Recording of Long-term Potentiation

In vivo Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices

Long-term Time Lapse Imaging of Mouse Cochlear Explants

Regioselective Biolistic Targeting in Organotypic Brain Slices Using a Modified Gene Gun

Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains

Agarose Microchambers for Long-term Calcium Imaging of Caenorhabditis elegans

A Simplified Method for Ultra-Low Density, Long-Term Primary Hippocampal Neuron Culture

Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method

Read Article