This content is Free Access.
1The Ph.D. Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology,Taipei Medical University, 2Integrated Laboratory, Center of Translational Medicine,Taipei Medical University, 3Core Facility,Taipei Medical University, 4The Ph.D. Program for Neural Regenerative Medicine, College of Medical Science and Technology,Taipei Medical University, 5Graduate Institute of Neural Regenerative Medicine, College of Medical Science and Technology,Taipei Medical University, 6TMU research center for Neurotrauma and Neuroregeneration, College of Medical Science and Technology,Taipei Medical University
Chapters
- 00:05Title
- 01:00Primary Neuron Culture and Brain Tissue Isolation
- 02:56Tissue Digestion, Plating, and Culture
- 04:32Live Image Recording
- 06:48Results: The Snapshots of GFP-GLUT4 in Hypothalamic Neurons
- 07:47Conclusion
Este protocolo describe una técnica para la observación de la fluorescencia verde en tiempo real la proteína (GFP) etiquetado proteína del transportador de glucosa 4 (GLUT4) trata sobre la estimulación de la insulina y la caracterización de la función biológica de CCR5 en la insulina, GLUT4 señalización vía con microscopía de deconvolución.
