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Live-Bilder von GLUT4 Protein Menschenhandel Maus primäre hypothalamische Neurone mit Dekonvolution Mikroskopie
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08:47 min
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December 07, 2017
DOI:
1The Ph.D. Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology,Taipei Medical University, 2Integrated Laboratory, Center of Translational Medicine,Taipei Medical University, 3Core Facility,Taipei Medical University, 4The Ph.D. Program for Neural Regenerative Medicine, College of Medical Science and Technology,Taipei Medical University, 5Graduate Institute of Neural Regenerative Medicine, College of Medical Science and Technology,Taipei Medical University, 6TMU research center for Neurotrauma and Neuroregeneration, College of Medical Science and Technology,Taipei Medical University
Summary
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Dieses Protokoll beschreibt eine Technik für die Beobachtung von Echtzeit-grüne Fluoreszenz Protein (GFP) Glucose Transporter 4 (GLUT4) Protein Menschenhandel auf Insulin-Stimulation und Charakterisierung der biologischen Rolle der CCR5 in Insulin-GLUT4 markiert Signalweg mit Dekonvolution Mikroskopie.
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Changou, C. A., Ajoy, R., Chou, S. Live Images of GLUT4 Protein Trafficking in Mouse Primary Hypothalamic Neurons Using Deconvolution Microscopy. J. Vis. Exp. (130), e56409, doi:10.3791/56409 (2017).
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