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Optical Screening of Novel Bacteria-specific Probes on Ex Vivo Human Lung Tissue by Confocal Laser Endomicroscopy
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Optical Screening of Novel Bacteria-specific Probes on Ex Vivo Human Lung Tissue by Confocal Laser Endomicroscopy

Optical Screening of Novel Bacteria-specific Probes on Ex Vivo Human Lung Tissue by Confocal Laser Endomicroscopy

7,294 Views

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09:24 min

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November 29, 2017

DOI:

10.3791/56284-v

DOI:
10.3791/56284-v

•

09:24 min
November 29, 2017

•

3 Views
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1EPSRC Proteus Hub, MRC Centre of Inflammation Research, Queen’s Medical Research Institute,University of Edinburgh, 2School of Chemistry, EaStChem,University of Edinburgh

Summary

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This technique describes an efficient screening process for evaluating bacteria-specific optical imaging agents within ex vivo human lung tissue, by fibered confocal fluorescence microscopy for the rapid identification of small molecule chemical probe-candidates with translatable potential.

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Optical ImagingConfocal Laser EndomicroscopyBacteria-specific ProbesMolecular ImagingImaging ProbesCalcein AM-labeled BacteriaFibered Confocal Fluorescence MicroscopyLaser Scanning UnitFCFM Imaging Fiber Calibration

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Cite this Article

Mills, B., Akram, A. R., Scholefield, E., Bradley, M., Dhaliwal, K. Optical Screening of Novel Bacteria-specific Probes on Ex Vivo Human Lung Tissue by Confocal Laser Endomicroscopy. J. Vis. Exp. (129), e56284, doi:10.3791/56284 (2017).

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