/

/

高分辨率荧光原位杂交在果蝇胚胎和组织中使用 Tyramide 信号放大

JoVE 杂志
遗传学

This content is Free Access.

JoVE 杂志遗传学

High Resolution Fluorescent In Situ Hybridization in Drosophila Embryos and Tissues Using Tyramide Signal Amplification

Please note that all translations are automatically generated. Click here for the English version.

高分辨率荧光原位杂交在果蝇胚胎和组织中使用 Tyramide 信号放大

12,492 Views

•

14:21 min

•

October 19, 2017

DOI:

10.3791/56281-v

DOI:

10.3791/56281-v

•

14:21 min

October 19, 2017

•

4 Views

Allison Jandura^1,2, Jack Hu¹, Ronit Wilk^1,2, Henry M. Krause^1,2

¹The Terrence Donnelly Centre for Cellular and Biomolecular Research,University of Toronto, ²Department of Molecular Genetics,University of Toronto

Summary

所描述的 rna原位杂交协议允许检测整个果蝇胚胎或解剖组织中的 rna。使用96井孔板和 tyramide 信号放大, 记录可以检测高分辨率, 灵敏度和吞吐量, 并以较低的成本。

Explore More Videos

Fluorescent In Situ Hybridization Drosophila RNA Tyramide Signal Amplification Subcellular Distribution High-throughput 96-well Plate PCR Bacterial Culture Plasmid DNA Ethanol Precipitation Centrifugation

Read Article