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在体内应用免疫模型检测抗原特异性 T 细胞细胞功能的试验
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
In Vivo Assay for Detection of Antigen-specific T-cell Cytolytic Function Using a Vaccination Model
DOI:

07:05 min

November 28, 2017

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Chapters

  • 00:05Title
  • 01:00Administration of the Immunostimulatory Cocktail Covax
  • 01:57Isolation of Target Splenocytes for Labeling with CFSE and Peptide Pulsing of Target Splenocytes
  • 02:55Preparation of CFSE and Labeling of Target Splenocytes
  • 04:52Results: Detection of Antigen-specific T-cell Cytolytic Function
  • 06:20Conclusion

Summary

自动翻译

该协议的目标是允许检测在小鼠模型中的靶细胞的体内抗原特异性杀伤。

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