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Cultivo In Vivo-gosto murino astrócitos usando o protocolo AWESAM rápida, simples e barato
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神经科学
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JoVE 杂志 神经科学
Culturing In Vivo-like Murine Astrocytes Using the Fast, Simple, and Inexpensive AWESAM Protocol

Cultivo In Vivo-gosto murino astrócitos usando o protocolo AWESAM rápida, simples e barato

8,209 Views

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07:56 min

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January 10, 2018

DOI:

10.3791/56092-v

DOI:
10.3791/56092-v

•

07:56 min
January 10, 2018

•

18 Views
,
1Chemical Biology, Chemistry Research Laboratory,University of Oxford, 2Department of Physiology, Anatomy, and Genetics,University of Oxford, 3Trans-synaptic signaling,European Neuroscience Institute

Summary

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O protocolo AWESAM descrito aqui é ideal para o cultivo de astrócitos murino em isolamento de outras células do cérebro de forma rápida, simples e barato. Astrócitos AWESAM exposição espontânea Ca2 + sinalização, morfologia e gene expressão perfis semelhantes de astrócitos na vivo.

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Cite this Article

Wolfes, A. C., Dean, C. Culturing In Vivo-like Murine Astrocytes Using the Fast, Simple, and Inexpensive AWESAM Protocol. J. Vis. Exp. (131), e56092, doi:10.3791/56092 (2018).

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