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Culturing In Vivo-like Murine Astrocytes Using the Fast, Simple, and Inexpensive AWESAM Protocol
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JoVE 杂志 神经科学
Culturing In Vivo-like Murine Astrocytes Using the Fast, Simple, and Inexpensive AWESAM Protocol
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Culturing In Vivo-like Murine Astrocytes Using the Fast, Simple, and Inexpensive AWESAM Protocol

DOI:
10.3791/56092-v

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07:56 min

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January 10, 2018

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,
1Chemical Biology, Chemistry Research Laboratory,University of Oxford, 2Department of Physiology, Anatomy, and Genetics,University of Oxford, 3Trans-synaptic signaling,European Neuroscience Institute

Chapters

  • 00:05Title
  • 00:39Rat or Mouse Brain Dissection
  • 02:50Tissue Digestion and Dissociation
  • 04:29Passaging Cells
  • 06:24Results: Spontaneous Ca2+ Signaling in Astrocytes
  • 07:12Conclusion

Summary

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The AWESAM protocol described here is optimal for culturing murine astrocytes in isolation from other brain cells in a fast, simple, and inexpensive manner. AWESAM astrocytes exhibit spontaneous Ca2+ signaling, morphology, and gene expression profiles similar to astrocytes in vivo.

Tags

In Vivo-like AstrocytesAWESAM ProtocolMurine Astrocyte CultureAstrocyte MonoculturesAstroglibiologyAstrocyte MorphologyAstrocyte MRNA And Protein ExpressionAstrocyte Calcium FluctuationsCortical Astrocyte IsolationHippocampal Astrocyte IsolationTrypsin-EDTA Treatment

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