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Horizontal Gel Electrophoresis for Enhanced Detection of Protein-RNA Complexes
Chapters
- 00:05Title
- 00:35Preparing the Horizontal Native Polyacrylamide Gel
- 02:27Binding Reaction
- 03:23Analysis of Samples
- 04:12Imaging the Gel
- 05:04Results: Horizontal Gel Electrophoresis Enhances the Detection of Distinct RNA-protein Complexes
- 06:08Conclusion
Native polyacrylamide gel electrophoresis is a fundamental tool for analyzing RNA-protein interactions. Traditionally most experiments have used vertical gels. However, horizontal gels provide several advantages, such as the opportunity to monitor complexes during electrophoresis. We provide a detailed protocol for generating and using horizontal native gel electrophoresis.
