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Detection of Residual Donor Erythroid Progenitor Cells after Hematopoietic Stem Cell Transplantation for Patients with Hemoglobinopathies
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发育生物学
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Detection of Residual Donor Erythroid Progenitor Cells after Hematopoietic Stem Cell Transplantation for Patients with Hemoglobinopathies

Detection of Residual Donor Erythroid Progenitor Cells after Hematopoietic Stem Cell Transplantation for Patients with Hemoglobinopathies

7,168 Views

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11:59 min

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September 06, 2017

DOI:

10.3791/56002-v

DOI:
10.3791/56002-v

•

11:59 min
September 06, 2017

•

17 Views
, , , ,
1Department of Pediatrics,Medical University Innsbruck, 2Department of Internal Medicine V (Hematology & Oncology),Medical University Innsbruck, 3Tyrolean Cancer Research Institute, 4Department of Pediatrics,Medical University Graz

Summary

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Quantification of donor-derived cells is required to monitor engraftment after stem cell transplantation in patients with hemoglobinopathies. A combination of flow cytometry-based cell sorting, colony formation assay, and subsequent analysis of short tandem repeats may be used to assess the proliferation and differentiation of progenitors in the erythroid compartment.

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Crazzolara, R., Kropshofer, G., Steurer, M., Sopper, S., Schwinger, W. Detection of Residual Donor Erythroid Progenitor Cells after Hematopoietic Stem Cell Transplantation for Patients with Hemoglobinopathies. J. Vis. Exp. (127), e56002, doi:10.3791/56002 (2017).

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