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Detection of Residual Donor Erythroid Progenitor Cells after Hematopoietic Stem Cell Transplantation for Patients with Hemoglobinopathies
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JoVE 杂志 发育生物学
Detection of Residual Donor Erythroid Progenitor Cells after Hematopoietic Stem Cell Transplantation for Patients with Hemoglobinopathies

Detection of Residual Donor Erythroid Progenitor Cells after Hematopoietic Stem Cell Transplantation for Patients with Hemoglobinopathies

DOI:
10.3791/56002-v

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11:59 min

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September 06, 2017

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, , , ,
1Department of Pediatrics,Medical University Innsbruck, 2Department of Internal Medicine V (Hematology & Oncology),Medical University Innsbruck, 3Tyrolean Cancer Research Institute, 4Department of Pediatrics,Medical University Graz

Chapters

  • 00:05Title
  • 01:22Isolation of Hematopoietic Bone Marrow cells by Multi-parameter Fluorescence-Actviated Cell Sorting (FACS)
  • 06:33Clonogenic Assay
  • 07:54Analysis of Chimerism by PCR
  • 09:16Results: Quantifying Relative Proportions of Donor-versus-recipient Chimerism in the Erythroid Progenitors
  • 10:54Conclusion

Summary

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Quantification of donor-derived cells is required to monitor engraftment after stem cell transplantation in patients with hemoglobinopathies. A combination of flow cytometry-based cell sorting, colony formation assay, and subsequent analysis of short tandem repeats may be used to assess the proliferation and differentiation of progenitors in the erythroid compartment.

Tags

Hematopoietic Stem Cell TransplantationHemoglobinopathiesChimerismErythroid Progenitor CellsFlow CytometryShort Tandem RepeatsGraft RejectionDisease RelapseImmunomodulatory StrategiesCell SuspensionBone MarrowGradient MediumMononuclear CellsCell ConcentrationTrypan Blue StainHemocytometer

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