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Determining Cell-surface Expression and Endocytic Rate of Proteins in Primary Astrocyte Cultures Using Biotinylation
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JoVE 杂志 神经科学
Determining Cell-surface Expression and Endocytic Rate of Proteins in Primary Astrocyte Cultures Using Biotinylation

Determining Cell-surface Expression and Endocytic Rate of Proteins in Primary Astrocyte Cultures Using Biotinylation

9,278 Views

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10:42 min

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July 03, 2017

DOI:

10.3791/55974-v

DOI:
10.3791/55974-v

•

10:42 min
July 03, 2017

•

4 Views
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1Department of Cellular and Physiological Sciences,University of British Columbia

Tags

Cell-surface BiotinylationPulse-chase BiotinylationAstrocytesAquaporin-4Transmembrane ProteinCell Surface ExpressionProtein InternalizationLamininWater Permeable ChannelPlasma MembraneCell CultureCell LysisProtein PurificationWestern Blot

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Tham, D. K. L., Moukhles, H. Determining Cell-surface Expression and Endocytic Rate of Proteins in Primary Astrocyte Cultures Using Biotinylation. J. Vis. Exp. (125), e55974, doi:10.3791/55974 (2017).

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