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JoVE Journal Neuroscience
Improving the Application of High Molecular Weight Biotinylated Dextran Amine for Thalamocortical Projection Tracing in the Rat
 

Improving the Application of High Molecular Weight Biotinylated Dextran Amine for Thalamocortical Projection Tracing in the Rat

Article DOI: 10.3791/55938-v • 06:39 min • April 12th, 2018
April 12th, 2018 •
*1, *1, 1, 1, 1, 1
1Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences
* These authors contributed equally

章节

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Automatic Translation

Here, we present a refined protocol to effectively reveal biotinylated dextran amine (BDA) labeling with a fluorescent staining method through a reciprocal neural pathway. It is suitable for analyzing the fine structure of BDA labeling and distinguishing it from other neural elements under a confocal laser scanning microscope.

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Keywords: Biotinylated Dextran Amine Neural Labeling Thalamocortical Projection Rat Neural Circuits Microsyringe Stereotaxic Craniotomy BDA Injection
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