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Peptide and Protein Quantification Using Automated Immuno-MALDI (iMALDI)
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Peptide and Protein Quantification Using Automated Immuno-MALDI (iMALDI)

Peptide and Protein Quantification Using Automated Immuno-MALDI (iMALDI)

DOI:
10.3791/55933-v

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08:57 min

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August 18, 2017

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, , , ,
1University of Victoria-Genome BC Proteomics Centre, 2Jewish General Hospital,McGill University, 3Dept of Biochemistry and Microbiology,University of Victoria, 4Proteomics Centre, Segal Cancer Centre, Lady Davis Institute, Jewish General Hospital,McGill University, 5Gerald Bronfman Department of Oncology,Jewish General Hospital

Chapters

  • 00:05Title
  • 01:06Generation of Ang I in Human Plasma
  • 03:01Immuno-enrichment of Ang I Using Antibody-coated Beads
  • 06:04Transfer of Beads onto MALDI Target Plate and MALDI-TOF-MS Analysis
  • 06:55Results: Quantification of Ang I Using iMALDI
  • 07:53Conclusion

Summary

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A protocol for the protein quantification in complex biological fluids using automated immuno-MALDI (iMALDI) technology is presented.

Tags

Keywords: Peptide QuantificationProtein QuantificationImmuno-MALDIAutomated AssayPlasma SamplesAngiotensin IAntibody ConjugationProtein BiomarkersQuantitative Proteomics

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