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Time-lapse konfokal billeddannelse af migrerende neuroner i organotypisk skive kultur af embryonale mus hjernen ved hjælp af<em> I Utero</em> Elektroporation
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Time-lapse Confocal Imaging of Migrating Neurons in Organotypic Slice Culture of Embryonic Mouse Brain Using In Utero Electroporation

Time-lapse konfokal billeddannelse af migrerende neuroner i organotypisk skive kultur af embryonale mus hjernen ved hjælp af<em> I Utero</em> Elektroporation

DOI:
10.3791/55886-v

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13:33 min

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July 25, 2017

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, , ,
1Institute of Molecular and Cellular Anatomy,Ulm University

Chapters

  • 00:05Title
  • 00:57In Utero Electroporation
  • 04:18Brain Dissection and Embedding for Organotypic Slice Culture
  • 06:40Vibratome Sectioning and Slice Culture
  • 08:47Ime-lapse Imaging
  • 10:30Results: Migration Behavior of Bcl11a Mutant Upper-layer Cortical Neurons
  • 12:40Conclusion

Summary

自动翻译

自动翻译

Denne protokol indeholder instruktioner til direkte observation af radialt migrerende kortikale neuroner. I utero- elektroporering, organotypisk skivekultur og time-lapse konfokal billeddannelse kombineres for direkte og dynamisk undersøgelse af virkningerne af overekspression eller nedregulering af gener af interesse for migrerende neuroner og for at analysere deres differentiering under udvikling.

Tags

Keywords: Time-lapse Confocal ImagingMigrating NeuronsOrganotypic Slice CultureEmbryonic Mouse BrainIn Utero ElectroporationNeocortex DevelopmentNeuron PolarizationRadial MigrationMigration SpeedMigratory DirectionPregnant MouseLateral VentricleMicroinjectionDNA SolutionTweezers-type Electrodes

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