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Isolation of the Side Population in Myc-induced T-cell Acute Lymphoblastic Leukemia in Zebrafish
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JoVE 杂志 免疫与感染
Isolation of the Side Population in Myc-induced T-cell Acute Lymphoblastic Leukemia in Zebrafish

Isolation of the Side Population in Myc-induced T-cell Acute Lymphoblastic Leukemia in Zebrafish

DOI:
10.3791/55711-v

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09:06 min

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May 04, 2017

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, , ,
1Department of Pediatrics, Section of Hematology-Oncology,The University of Chicago, 2Department of Biology and Biotechnology,Worcester Polytechnic Institute

Chapters

  • 00:05Title
  • 01:07Isolation of Fluorescently Labeled T-ALL Cells from Zebrafish
  • 04:01Identification of Side Population Cells in Zebrafish T-ALL
  • 06:53Results: Side Population Identification in T-ALL Cells from Zebrafish
  • 08:01Conclusion

Summary

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Here, we describe a technique to isolate the side population cells from a zebrafish model of myc-induced T-cell acute lymphoblastic leukemia (T-ALL). This side population assay is highly sensitive and is described for zebrafish T-ALL, but it may be applicable to other malignant and non-malignant zebrafish cell types.

Tags

Keywords: Side PopulationT-cell Acute Lymphoblastic LeukemiaZebrafishMyc-induced CancerCancer Stem CellsCell IsolationHeparinEuthanasiaFBS/PBSCell Collection

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