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背根神经节内注射和背根挤压伤为感官轴突再生模型
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Dorsal Root Ganglion Injection and Dorsal Root Crush Injury as a Model for Sensory Axon Regeneration

背根神经节内注射和背根挤压伤为感官轴突再生模型

DOI:

09:48 min

May 03, 2017

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Chapters

  • 00:05Title
  • 00:48Preoperative Procedures
  • 01:45DRG Injection and Dorsal Root Crush Injury
  • 05:08Postoperative Procedures
  • 06:34Anterograde CTB Injection for Axonal Tracing
  • 07:25Results: Sensory Axon Regeneration Visualized with GFP and CTB after Dorsal Root Crush Injury
  • 08:59Conclusion

Summary

自动翻译

This protocol presents the use of a dorsal root ganglion (DRG) injection with a viral vector and a concurrent dorsal root crush injury in an adult rat as a model to study sensory axon regeneration. This model is suitable for investigating the use of gene therapy to promote sensory axon regeneration.

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