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1Department of Medicine, General Pathology Division, Cystic Fibrosis Translational Research Laboratory “D. Lissandrini”,University of Verona, 2Corporate Preclinical R&D,Chiesi Farmaceutici S.p.A., 3Department of Diagnostic and Public Health, Microbiology Division,University of Verona, 4Dipartimento di Scienze Medico-Veterinarie,University of Parma, 5Department of Biomedical Biotechnological and Translational Sciences,University of Parma, 6Department of Computer Science,University of Verona, 7Department of Neurological, Biomedical and Movement Sciences,University of Verona, 8Cystic Fibrosis Regional Center (CFC),AOUI Verona
Chapters
- 00:05Title
- 01:07In Vivo Gene Delivery and Biomluminescent Imaging (BLI) and Photon Quantitification
- 04:24Pro-inflammatory Stimuli Mouse Challenge
- 06:51Results: Representative P. aeruginosa-induced Lung Inflammation Supernatent In Vivo Imaging in IL-8 Transiently Trangenic Mice
- 07:42Conclusion
O método aqui descrito permite a visualização da ativação da inflamação dependente do promotor de IL-8 nos pulmões de camundongos através de imagem de bioluminescência não-invasiva (BLI). O mesmo animal pode ser submetido a BLI várias vezes por até dois meses a partir do momento da entrega da construção repórter de luciferase.
