需要订阅 JoVE 才能查看此. 登录或开始免费试用。
チャンネルロドプシンにおけるイオン選択性の電気生理学的測定のための全細胞パッチクランプ記録
16,641 Views
•
08:39 min
•
May 22, 2017
DOI:
Summary
Automatically generated
This article describes how the ion selectivity of channelrhodopsin is determined with electrophysiological whole-cell patch-clamp recordings using HEK293 cells. Here, the experimental procedure for investigating chloride selectivity of an anion-selective channelrhodopsin is demonstrated. However, the procedure is transferable to other channelrhodopsins of distinct selectivity.
Read Article
Cite this Article
Grimm, C., Vierock, J., Hegemann, P., Wietek, J. Whole-cell Patch-clamp Recordings for Electrophysiological Determination of Ion Selectivity in Channelrhodopsins. J. Vis. Exp. (123), e55497, doi:10.3791/55497 (2017).
Copy