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Assessment of Neuronal Viability Using Fluorescein Diacetate-Propidium Iodide Double Staining in Cerebellar Granule Neuron Culture
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JoVE 杂志 神经科学
Assessment of Neuronal Viability Using Fluorescein Diacetate-Propidium Iodide Double Staining in Cerebellar Granule Neuron Culture

Assessment of Neuronal Viability Using Fluorescein Diacetate-Propidium Iodide Double Staining in Cerebellar Granule Neuron Culture

DOI:
10.3791/55442-v

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08:53 min

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May 10, 2017

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, , , , , , , , ,
1Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine,Ningbo University, 2Department of Applied Biology and Chemistry Technology, Institute of Modern Chinese Medicine,The Hong Kong Polytechnic University, 3Institute of New Drug Research, Guangdong Provincial Key Laboratory of Pharmacodynamic, Constituents of Traditional Chinese Medicine & New Drug Research, College of Pharmacy,Jinan University, 4International Joint Laboratory (SYSU-PolyU HK) of Novel Anti-Dementia Drugs of Guangdong

Chapters

  • 00:05Title
  • 00:46Tissue Harvest from 8 Day Old Sprague-Dawley Rats
  • 03:51FDA-PI Double Staining and FDA-PI-Hoechst Triple Staining in Cerebellar Granule Neuron Culture
  • 05:30Assessment of Neuronal Viability
  • 07:08Results: Double and Triple Stainings in CGN Culture
  • 08:19Conclusion

Summary

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This protocol describes how to accurately measure neuronal viability using Fluorescein diacetate (FDA) and Propidium Iodide (PI) double staining in cultured cerebellar granule neurons, a primary neuronal culture used as an in vitro model in neuroscience and neuropharmacology research.

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Neuronal ViabilityFluorescein Diacetate-propidium IodideCerebellar Granule Neuron CultureCell Viability神经科学NeuropharmacologyGlial CellsNeuronsMISTA Cell CultureRat PupsCerebellumDissectionCentrifugationTrypsinDNaseSoybean Trypsin InhibitorMagnesium SulfateCalcium ChlorideCell Density6-well PlateCell Culture

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