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A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography (HPLC)
JoVE 杂志
化学
This content is Free Access.
JoVE 杂志 化学
A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography (HPLC)

A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography (HPLC)

DOI:
10.3791/55425-v

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10:09 min

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March 15, 2017

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,
1German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, 2Institute of Ecology,Friedrich Schiller University Jena

Chapters

  • 00:05Title
  • 01:04Extraction Preparation
  • 04:03Glucosinolate Extraction and Analysis
  • 08:02Results: Identification and Quantification of Extracted Glucosinolates
  • 09:10Conclusion

Summary

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Here, we describe in great detail an established and robust protocol for the extraction of glucosinolates from ground plant materials. After an on-column sulfatase treatment of the extracts, the desulfoglucosinolates are eluted and analyzed by high-pressure liquid chromatography.

Tags

Keywords: GlucosinolateExtractionHPLCPlant-insect EcologyPlant PathologyPlant BreedingFood SciencesG-25 Dextrin GelArylsulfataseEthanolCentrifugationSinigrinReference SolutionsFreeze-dryingMicrocentrifuge Tubes

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