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A Device for Performing Cell Migration/Wound Healing in a 96-Well Plate
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发育生物学
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JoVE 杂志 发育生物学
A Device for Performing Cell Migration/Wound Healing in a 96-Well Plate

A Device for Performing Cell Migration/Wound Healing in a 96-Well Plate

DOI:
10.3791/55411-v

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05:46 min

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March 07, 2017

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, ,
1Department of Biology,Hong Kong Baptist University

Chapters

  • 00:05Title
  • 00:59Introducing the Wounder Parts
  • 01:38Setting the Pin Holder Width
  • 02:05Adjusting the Guiding Bars
  • 02:49Calibrating the Pins
  • 03:23Scratching the Cell Monolayer
  • 04:44Results: Uniform Wound Width with Device
  • 05:13Conclusion

Summary

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Here, we present a protocol to perform a semi-high throughput cell migration assay on a 96-well cell culture plate. This protocol is a fast, simple and economical method to create consistent scratch wounds on a cell monolayer.

Tags

Cell MigrationWound Healing96-well PlateIn Vitro AssayCell Migration QuantificationSemi-high ThroughputAdjustable Wounding PinsGuiding BarPipet TipsHex ScrewHex Socket Head CapsAdjusting Rings

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