Journal
/
神经科学
/
Organotypic hippocampus skive kulturer som en Model til undersøgelse Neuroprotection og invasionsevne af tumorceller
JoVE 杂志
神经科学
Author Produced
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 神经科学
Organotypic Hippocampal Slice Cultures As a Model to Study Neuroprotection and Invasiveness of Tumor Cells

Organotypic hippocampus skive kulturer som en Model til undersøgelse Neuroprotection og invasionsevne af tumorceller

DOI:
10.3791/55359-v

•

07:48 min

•

August 27, 2017

•

, , ,
1Department of Anatomy and Cell Biology,Martin Luther University Halle-Wittenberg, 2Department of Anatomy,University of Otago

Chapters

  • 00:00Title
  • 00:08Agar Preparation
  • 00:54Brain Fixation
  • 02:29Slicing Procedure
  • 03:26Hippocampus Removal
  • 04:05Culturing Method
  • 05:18Representative Results
  • 07:19Conclusion

Summary

自动翻译

自动翻译

Organotypic hippocampus skive kulturer (OHSC) repræsenterer en in vitro- model, der simulerer i vivo situationen meget godt. Her beskriver vi en vibratome-baseret forbedret udskæring protokol for at opnå høj kvalitet skiver til brug ved vurderingen af neuroprotektive potentiale af nye stoffer eller den biologiske opførsel af tumorceller.

Tags

Keywords: Organotypic Hippocampal Slice CulturesNeuroprotectionTumor Cell InvasivenessVibratomeAgar BlocksPreparation MediumCytoarchitectureCell Culture InsertCulture MediumInflammatory ReactionsNeuroprotection Studies

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

check_url/cn/55359?article_type=v

The Organotypic Hippocampal Slice Culture Model for Examining Neuronal Injury
check_url/cn/55359?article_type=v

Organotypic Hippocampal Slice Cultures
check_url/cn/55359?article_type=v

Organotypic Cerebellar Cultures: Apoptotic Challenges and Detection
check_url/cn/55359?article_type=v

Organotypiske Slice Kulturer af Embryonale ventrale midthjernen: et system til undersøgelse Dopaminerge Neuronal Udvikling In vitro</em
check_url/cn/55359?article_type=v

The Analysis of Purkinje Cell Dendritic Morphology in Organotypic Slice Cultures
check_url/cn/55359?article_type=v

Post-embedding immunogold mærkning af synaptiske proteiner i hippokampalskivekulturer
check_url/cn/55359?article_type=v

Organotypiske skivekulturer at studere oligodendrocyt Dynamics og myelinering
check_url/cn/55359?article_type=v

Analysen af ​​neurovaskulære Remodeling i Entorhino-hippocampus organotypisk skivekulturer
check_url/cn/55359?article_type=v

Forberedelse og immunfarvning af Myelinating Organotypic cerebellare skive kulturer
check_url/cn/55359?article_type=v

Purkinje-cellernes overlevelse i Udsnitskulturer af Organotypiske cerebellar

Read Article