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Multimer-PAGE: A Method for Capturing and Resolving Protein Complexes in Biological Samples
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
Multimer-PAGE: A Method for Capturing and Resolving Protein Complexes in Biological Samples

Multimer-PAGE: A Method for Capturing and Resolving Protein Complexes in Biological Samples

DOI:
10.3791/55341-v

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07:40 min

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May 05, 2017

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, , ,
1Physiology,Michigan State University, 2Center for Neurodegenerative Science Van Andel Institute, 3Pharmaceutical Sciences,Wayne State University

Chapters

  • 00:05Title
  • 00:45Cross-linking
  • 02:23Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)
  • 05:40Results: Cross-linking and Detection of Complexes in Rat Brain Homogenate
  • 06:46Conclusion

Summary

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A method for stabilizing and separating native protein complexes from unmodified tissue lysate using an amine-reactive protein cross-linker coupled to a novel two-dimensional polyacrylamide gel electrophoresis (PAGE) system is presented.

Tags

Protein ComplexesNative ConditionsBlue Native Polyacrylamide Gel ElectrophoresisDSP CrosslinkingSDS-PAGEProtein IdentificationProtein Analysis

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