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Dissection of Larval Zebrafish Gonadal Tissue
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JoVE 杂志 发育生物学
Dissection of Larval Zebrafish Gonadal Tissue

Dissection of Larval Zebrafish Gonadal Tissue

DOI:
10.3791/55294-v

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10:43 min

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April 26, 2017

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, , , , ,
1Institutes of Brain Science, State Key Laboratory of Medical Neurobiology and Collaborative Innovation Center for Brain Science,Fudan University

Chapters

  • 00:05Title
  • 01:03Experimental Preparation
  • 03:31Dissection of Gonadal Tissue from 17 and 25 dpf Larvae
  • 05:46Analyze Gene Expression of the Isolated Gonadal Tissues
  • 08:39Results: Gonadal Tissue Dissection in Zebrafish Larvae
  • 09:46Conclusion

Summary

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Here, we present a protocol for isolating gonadal tissue of larval zebrafish, which will facilitate investigations of zebrafish sex differentiation and maintenance.

Tags

ZebrafishLarval DissectionSex DevelopmentMolecular BiologyAgar PlateAnesthesiaRinger’s SolutionStereomicroscope

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