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Effektiv Differentiering af pluripotente stamceller til NKX6-1<sup> +</sup> Pankreatisk progenitorer
JoVE 杂志
发育生物学
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JoVE 杂志 发育生物学
Efficient Differentiation of Pluripotent Stem Cells to NKX6-1+ Pancreatic Progenitors
DOI:

09:23 min

March 07, 2017

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Chapters

  • 00:05Title
  • 01:01HESC Differentiation and Flow Cytometry Analysis
  • 01:28Harvesting Cells for Flow Cytometry Analysis
  • 03:17Flow Preparation for Live Staining on Day 3
  • 04:41Flow Preparation for Fixed Staining on Day 13
  • 07:04Results: Schematic of Pancreatic Progenitor Differentiation from hESCs and Supporting Data
  • 08:26Conclusion

Summary

自动翻译

Her beskriver vi en 4-trins-protokol til at differentiere humane embryonale stamceller til NKX6-1 + bugspytkirtlen forfædre in vitro. Denne protokol kan anvendes på en række humane pluripotente stamcellelinjer.

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