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Conditional Reprogramming of Pediatric Human Esophageal Epithelial Cells for Use in Tissue Engineering and Disease Investigation
Chapters
- 00:05Title
- 01:213T3 Feeder Cell Culture and Irradiation
- 02:31Pediatric Esophageal Biospy Acquisition and Processing
- 05:00Cell Culture Expansion, Freezing, and Storage
- 07:17Results: Representative Human Esophageal Cell Characterization
- 08:59Conclusion
Expansion of human pediatric esophageal epithelial cells utilizing conditional reprogramming provides investigators with a patient-specific population of cells that can be utilized for engineering esophageal constructs for autologous implantation to treat defects or injury and serve as a reservoir for therapeutic screening assays.
