Journal
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发育生物学
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Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta
JoVE 杂志
发育生物学
This content is Free Access.
JoVE 杂志 发育生物学
Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta

Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta

DOI:
10.3791/55224-v

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07:06 min

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April 03, 2017

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, , , , , ,
1Department of Biological Sciences,Oakland University, 2OU-WB Institute for Stem Cell and Regenerative Medicine, 3Department of Obstetrics and Gynecology,St. John Provindence – Providence Park Hospital, 4Department of Neurosurgery,Beaumont Health System

Chapters

  • 00:05Title
  • 01:09Human Umbilical Cord (UC), Cord-Placenta Junction (CPJ), and Fetal Placenta (FP) Processing and Cell Isolation
  • 04:29Results: Colony-Forming Efficiency and Mesenchmyal Stromal Cell (MSC) Marker Expression of Perinatal Tissue-Isolated Cells
  • 06:21Conclusion

Summary

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Here, we present a protocol for the dissection of human umbilical cord (UC) and fetal placenta sample into cord lining (CL), Wharton's jelly (WJ), cord-placenta junction (CPJ), and fetal placenta (FP) for the isolation and characterization of mesenchymal stromal cells (MSCs) using the explant culture technique.

Tags

Mesenchymal Stromal CellsUmbilical CordFetal PlacentaStem Cell BiologyAlternative MedicineMulti-potentCord Mesenchymal Stromal CellsAnatomical RegionsUmbilical CordWharton’s JellyEpitheliumSubamnionBlood VesselsPerivascular JellyTrypsinPartial Digestion

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